EDNRB/β-Arrestin/CHO
CBP71376
詢 價
索取protocol
產品描述
產品數(shù)據(jù)庫
| I. Background | |
| EDNRB (Endothelin Receptor Type B) is a Protein Coding gene. Diseases associated with EDNRB include Waardenburg Syndrome, Type 4A and Abcd Syndrome. Among its related pathways are Class A/1 (Rhodopsin-like receptors) and Endothelins. Gene Ontology (GO) annotations related to this gene include G protein-coupled receptor activity and type 1 angiotensin receptor binding. | |
| II. Introduction | |
| Host Cell: |
CHO |
| Stability: | 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K+10%FBS+5ug/ml puromycin+5ug/ml blasticidin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): |
Functional assay for EDNRB |
| Transducer: | β-Arrestin |
| Ⅲ. Description of Host Cell Line | |
| Organism: | Hamster |
| Tissue: | Ovary |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| Ⅳ. Representative Data | |
|
Figure 1.Recombinant EDNRB/β-Arrestin/CHO constitutively expressing EDNRB/β-Arrestin. |
|
|
Figure 2. Dose response of Endothelin 3 (human,mouse,rabbit, rat TFA) in CHO-K1 EDNRB(ET2,ETB) β-Arrestin Cell line（C11）. |
|
